Die Biomasse (170 g) mit berexprimiertem GST wurde in 1,7 l Lysepuffer lysiert
In Methods in Enzymology
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Slides were dried for at least 20 hours at 4C and fluorescent images were obtained with Slidebook 4.2 Digital Microscopy Software driving an Olympus 1X81 inverted confocal microscope with a 60X oil immersion lens, NA 1.4 and resolution 0.280 Dendritic spine density was measured on primary and secondary dendrites at a distance of at least 150 from the soma
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What you need on hand: The lyophilized peptide vial The correct solvent (the one named on the certificate of analysis) A fresh sterile syringe and needle for each draw Alcohol wipes and an alcohol-wiped work surface A labeled storage spot at 2 to 8C The full sequence, start to finish: Bring the sealed peptide vial and solvent to room temperature so condensation does not cloud the solution
